HalophFGD
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Primer Design
Powered by
Primer3
Paste or upload a DNA sequence (single FASTA, ≥ 50 bp). Primer design is powered by
Primer3
, and parameters follow its conventions.
DNA sequence (paste or FASTA)
Fill example
Supports A/C/G/T/N. If a file is uploaded, the file content takes precedence.
Or upload FASTA file
Product size (bp)
-
Number of pairs:
Primer length (nt)
Min
Opt
Max
Tm (°C)
Min
Opt
Max
GC content (%)
Min
Max
Species for BLAST specificity (optional)
Apium graveolens
Asparagus officinalis
Atriplex hortensis
Avicennia marina
Beta vulgaris
Brassica nigra
Bruguiera sexangula
Carallia pectinifolia
Casuarina equisetifolia
Casuarina glauca
Ceriops tagal
Ceriops zippeliana
Chenopodium album
Chenopodium quinoa
Cocos nucifera
Cymodocea nodosa
Dunaliella salina
Echinochloa crus-galli
Eleusine coracana
Eutrema salsugineum
Flaveria linearis
Flaveria trinervia
Hibiscus hamabo
Kandelia candel
Limonium bicolor
Lolium multiflorum
Lycium barbarum
Mesembryanthemum crystallinum
Nitraria sibirica
Oryza coarctata
Paspalum vaginatum
Phoenix dactylifera
Pistacia vera
Plantago ovata
Populus euphratica
Portulaca oleracea
Posidonia oceanica
Puccinellia tenuiflora
Reaumuria soongarica
Rhizophora apiculata
Rhizophora mangle
Salicornia bigelovii
Salicornia europaea
Schrenkiella parvula
Solanum chilense
Solanum pennellii
Sporobolus alterniflorus
Suaeda aralocaspica
Suaeda glauca
Tamarix chinensis
Thalassia testudinum
Thinopyrum elongatum
Triticum dicoccoides
Zostera marina
Zoysia japonica
Zoysia macrostachya
Used later for BLAST specificity database selection; does not affect Primer3 constraints.
Target region (optional)
start (0-based)
length
Example: start=100, length=50 →
SEQUENCE_TARGET=100,50
Submit
Text ≤ 100 lines; file ≤ 300 KB
Primer task has been submitted