HalophFGD

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Primer Design Powered by Primer3

Paste or upload a DNA sequence (single FASTA, ≥ 50 bp). Primer design is powered by Primer3, and parameters follow its conventions.
Supports A/C/G/T/N. If a file is uploaded, the file content takes precedence.
-
Number of pairs:
Min
Opt
Max
Min
Opt
Max
Min
Max
Used later for BLAST specificity database selection; does not affect Primer3 constraints.
start (0-based)
length
Example: start=100, length=50 → SEQUENCE_TARGET=100,50
Text ≤ 100 lines; file ≤ 300 KB
Maintained by Hengyu Yan - College of Agronomy - Qingdao Agricultural University © 2024 All Rights Reserved.
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