Basic Information
Locus ID:
AsparagusV1_03.698.V1.1
Species & Taxonomic ID:
Asparagus officinalis & 4686
Genome Assembly:
GCA_001876935.1
Description:
Cytochrome P450
Maps and Mapping Data
| Chromosome | Start | End | Strand | ID |
|---|---|---|---|---|
| chr3 | 15043026 | 15103704 | + | AsparagusV1_03.698.V1.1 |
Protein Data
Protein Properties:
| Theoretical pI | Molecular Weight | Instability Index | Aliphatic Index | GRAVY |
|---|---|---|---|---|
| 6.75 | 89,393.12 Da | 46.03 | 86.45 | -0.30 |
Protein Domain:
| Category | ID | Description | Start | End | Evalue/Score | InterPro ID |
|---|---|---|---|---|---|---|
| CDD | cd00170 | SEC14 | 585 | 725 | 3.64606E-38 | IPR001251 |
| Pfam | PF00650 | CRAL/TRIO domain | 574 | 725 | 5.7E-34 | IPR001251 |
| Pfam | PF03765 | CRAL/TRIO, N-terminal domain | 526 | 551 | 1.1E-6 | IPR011074 |
| Pfam | PF00067 | Cytochrome P450 | 37 | 345 | 3.3E-21 | IPR001128 |
| Pfam | PF00067 | Cytochrome P450 | 455 | 489 | 8.7E-7 | IPR001128 |
| Pfam | PF00067 | Cytochrome P450 | 346 | 434 | 1.8E-21 | IPR001128 |
| SUPERFAMILY | SSF48264 | Cytochrome P450 | 32 | 432 | 2.62E-75 | IPR036396 |
| SUPERFAMILY | SSF48264 | Cytochrome P450 | 459 | 499 | 1.31E-8 | IPR036396 |
| SUPERFAMILY | SSF46938 | CRAL/TRIO N-terminal domain | 496 | 568 | 2.88E-17 | IPR036273 |
| SUPERFAMILY | SSF52087 | CRAL/TRIO domain | 566 | 744 | 1.44E-47 | IPR036865 |
| Gene3D | G3DSA:1.10.630.10 | Cytochrome P450 | 342 | 454 | 2.8E-25 | IPR036396 |
| Gene3D | G3DSA:1.10.630.10 | Cytochrome P450 | 25 | 341 | 5.5E-59 | IPR036396 |
| Gene3D | G3DSA:3.40.525.10 | - | 478 | 764 | 6.4E-80 | IPR036865 |
| SMART | SM00516 | sec14_4 | 572 | 727 | 6.1E-48 | IPR001251 |
| SMART | SM01100 | CRAL_TRIO_N_2 | 527 | 552 | 7.4E-7 | IPR011074 |
| ProSiteProfiles | PS50191 | CRAL-TRIO lipid binding domain profile. | 585 | 730 | 19.971189 | IPR001251 |
| ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 462 | 471 | - | IPR017972 |
| ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 395 | 404 | - | IPR017972 |
| PRINTS | PR00385 | P450 superfamily signature | 402 | 413 | 1.3E-5 | IPR001128 |
| PRINTS | PR00385 | P450 superfamily signature | 287 | 304 | 1.3E-5 | IPR001128 |
| PRINTS | PR00385 | P450 superfamily signature | 393 | 402 | 1.3E-5 | IPR001128 |
| PRINTS | PR00463 | E-class P450 group I signature | 276 | 293 | 1.3E-18 | IPR002401 |
| PRINTS | PR00463 | E-class P450 group I signature | 296 | 322 | 1.3E-18 | IPR002401 |
| PRINTS | PR00463 | E-class P450 group I signature | 69 | 88 | 1.3E-18 | IPR002401 |
| PRINTS | PR00463 | E-class P450 group I signature | 402 | 425 | 1.3E-18 | IPR002401 |
| PRINTS | PR00463 | E-class P450 group I signature | 392 | 402 | 1.3E-18 | IPR002401 |
| PRINTS | PR00463 | E-class P450 group I signature | 357 | 381 | 1.3E-18 | IPR002401 |
| Coils | Coil | Coil | 240 | 260 | - | - |
Gene Ontology
KEGG Pathway
Pathway:
Reaction:
R07430 (6-Oxocampestanol + Oxygen + NADPH <=> Cathasterone + NADP+ + H2O)
R07445 (Campesterol + Oxygen + [Reduced NADPH---hemoprotein reductase] <=> 22alpha-Hydroxy-campesterol + [Oxidized NADPH---hemoprotein reductase] + H2O)
R07452 (Campest-4-en-3-one + Oxygen + [Reduced NADPH---hemoprotein reductase] <=> (22S)-22-Hydroxycampest-4-en-3-one + [Oxidized NADPH---hemoprotein reductase] + H2O)
R07453 (5alpha-Campestan-3-one + Oxygen + [Reduced NADPH---hemoprotein reductase] <=> 22alpha-Hydroxy-5alpha-campestan-3-one + [Oxidized NADPH---hemoprotein reductase] + H2O)
R07454 (Campestanol + Oxygen + [Reduced NADPH---hemoprotein reductase] <=> 6-Deoxocathasterone + [Oxidized NADPH---hemoprotein reductase] + H2O)
Best hit
| Source | Best Hit ID | Description | E-value |
|---|---|---|---|
| TAIR | AT3G50660.1 | Cytochrome P450 superfamily protein. Encodes a 22α hydroxylase whose reaction is a rate-limiting step in brassinosteroid biosynthetic pathway. The protein is a member of CYP90B gene family. CLM is an epi-allele with small, compressed rosette, reduced internode length, and reduced fertility, appears in selfed ddm mutant plants possibly due to loss of cytosine methylation. Transcripts accumulate in actively growing tissues, and GUS expression is negatively regulated by brassinosteroids. Localized in the endoplasmic reticulum. The in vitro expressed protein can perform the C-22 hydroxylation of a variety of C27-, C28- and C29-sterols. Cholesterol was the best substrate, followed by campesterol. Sitosterol was a poor substrate. | 0 |
| RefSeq | XP_020256267.1 | cytochrome P450 90B1-like [Asparagus officinalis] | 0 |
| A0A5A4DV62 | Cholesterol 22-hydroxylase CYP90B27 OS=Paris polyphylla OX=49666 GN=CYP90B27 PE=1 SV=1 | 0 | |
| TrEMBL | A0A5P1FAQ6 | CRAL-TRIO domain-containing protein OS=Asparagus officinalis OX=4686 GN=A4U43_C03F6980 PE=4 SV=1 | 0 |
Expression
| BioProject | Accession | TPM | Cultivar | Tissue | Development Stage | Sample Name | Description |
|---|---|---|---|---|---|---|---|
| No sample metadata found. | |||||||
Orthology