halophFGD

Basic Information

Locus ID: Ag7G00136

Species & Taxonomic ID: Apium graveolens & 4045

Genome Assembly: GCA_009905375.1

Description: Belongs to the helicase family. RecQ subfamily

Maps and Mapping Data

Chromosome	Start	End	Strand	ID
chr7	3929176	3941217	+	Ag7G00136

Protein Data

Protein Properties:

Theoretical pI	Molecular Weight	Instability Index	Aliphatic Index	GRAVY
7.89	89,541.23 Da	46.42	91.06	-0.17

Protein Domain:

Category	ID	Description	Start	End	Evalue/Score	InterPro ID
CDD	cd17920	DEXHc_RecQ	2	196	2.46525E-92	-
CDD	cd18794	SF2_C_RecQ	197	332	1.1481E-52	-
Pfam	PF14493	Helix-turn-helix domain	648	744	2.8E-16	IPR029491
Pfam	PF16124	RecQ zinc-binding	335	397	2.3E-9	IPR032284
Pfam	PF00570	HRDC domain	542	607	7.4E-15	IPR002121
Pfam	PF00271	Helicase conserved C-terminal domain	218	322	1.6E-21	IPR001650
Pfam	PF09382	RQC domain	399	496	7.7E-22	IPR018982
Pfam	PF00270	DEAD/DEAH box helicase	16	176	5.5E-20	IPR011545
SUPERFAMILY	SSF46785	Winged helix DNA-binding domain	395	498	1.26E-23	IPR036390
SUPERFAMILY	SSF47819	HRDC-like	540	611	1.09E-15	IPR010997
SUPERFAMILY	SSF52540	P-loop containing nucleoside triphosphate hydrolases	21	334	1.44E-54	IPR027417
Gene3D	G3DSA:1.10.150.80	HRDC domain	540	610	2.7E-16	IPR044876
Gene3D	G3DSA:3.40.50.300	-	197	398	4.4E-55	IPR027417
Gene3D	G3DSA:1.10.10.10	-	399	520	3.4E-31	IPR036388
Gene3D	G3DSA:3.40.50.300	-	1	195	6.0E-74	IPR027417
SMART	SM00341	hrdc7	537	617	2.2E-5	IPR002121
SMART	SM00487	ultradead3	10	201	4.6E-30	IPR014001
SMART	SM00956	RQC_2	404	508	2.1E-19	IPR018982
SMART	SM00490	helicmild6	242	323	6.8E-27	IPR001650
TIGRFAM	TIGR00614	recQ_fam: ATP-dependent DNA helicase, RecQ family	3	455	6.8E-149	IPR004589
ProSiteProfiles	PS51192	Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile.	22	189	23.964119	IPR014001
ProSiteProfiles	PS51194	Superfamilies 1 and 2 helicase C-terminal domain profile.	217	369	19.108353	IPR001650
ProSiteProfiles	PS50967	HRDC domain profile.	537	617	17.583952	IPR002121

Gene Ontology

Biological Process:

GO:0006260 (DNA replication) GO:0006281 (DNA repair) GO:0006310 (DNA recombination) GO:0008152 (metabolic process)

Molecular Function:

GO:0000166 (nucleotide binding) GO:0003676 (nucleic acid binding) GO:0004386 (helicase activity) GO:0005524 (ATP binding) GO:0043138 (3'-5' DNA helicase activity)

KEGG Pathway

KO Term:

K03654 (ATP-dependent DNA helicase RecQ [EC:5.6.2.4])

Pathway:

ko03018 (RNA degradation) map03018 (RNA degradation)

Best hit

Source	Best Hit ID	Description
TAIR	AT1G31360.4	-
RefSeq	XP_028121142.1	uncharacterized protein LOC114318451 isoform X1 [Camellia sinensis]
Swiss-Prot	P71359	ATP-dependent DNA helicase RecQ OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=recQ PE=3 SV=1
TrEMBL	A0A5B7A5X2	ATP-dependent DNA helicase (Fragment) OS=Davidia involucrata OX=16924 GN=Din_021611 PE=3 SV=1

Expression

BioProject	Accession	TPM	Cultivar	Tissue	Development Stage	Sample Name	Description
PRJNA723697	SRX10692252	44.593971	Shengqin NO.2	seed	S1-1	S1-1	initial formation stage (S1)
PRJNA723697	SRX10692254	15.269659	Shengqin NO.2	seed	S1-3	S1-3	initial formation stage (S1)
PRJNA723697	SRX10692255	15.536059	Shengqin NO.2	seed	S3-1	S3-1	middle development stage (S3)
PRJNA723697	SRX10692256	13.753577	Shengqin NO.2	seed	S3-2	S3-2	middle development stage (S3)
PRJNA723697	SRX10692257	13.386107	Shengqin NO.2	seed	S3-3	S3-3	middle development stage (S3)
PRJNA723697	SRX10692258	11.878559	Shengqin NO.2	seed	S5-1	S5-1	maturation stage (S5)
PRJNA723697	SRX10692259	22.501617	Shengqin NO.2	seed	S5-2	S5-2	maturation stage (S5)
PRJNA723697	SRX10692260	20.590517	Shengqin NO.2	seed	S5-3	S5-3	maturation stage (S5)
PRJNA884666	SRX17729272	10.535379	celery	anther		W99A-r1	male sterile lineÂ
PRJNA884666	SRX17729273	10.952142	celery	anther		W99A-r2	male sterile lineÂ
PRJNA884666	SRX17729274	13.189745	celery	anther		W99A-r3	male sterile lineÂ
PRJNA884666	SRX17729275	11.587656	celery	anther		W99B-r1	maintainer line
PRJNA884666	SRX17729276	9.939817	celery	anther		W99B-r2	maintainer line
PRJNA884666	SRX17729277	7.595403	celery	anther		W99B-r3	maintainer line
PRJNA884180	SRX17743889	2.571606	var. secalinum Yablochny	collenchyma		Col_S1_rep1-1
PRJNA884180	SRX17748056	1.060637	var. secalinum Yablochny	collenchyma		Col_S1_rep3	biological replicate 3
PRJNA884180	SRX17748057	3.227658	var. secalinum Yablochny	collenchyma		Col_S1_rep4	biological replicate 4
PRJNA884180	SRX17748678	10.235584	var. secalinum Yablochny	parenchyma		Par_S1_rep1	Biological replicate 1
PRJNA884180	SRX17748679	1.588466	var. secalinum Yablochny	parenchyma		Par_S1_rep2	Biological replicate 2
PRJNA884180	SRX17748680	1.163412	var. secalinum Yablochny	parenchyma		Par_S1_rep3	Biological replicate 3
PRJNA884180	SRX17748681	2.239238	var. secalinum Yablochny	parenchyma		Par_S1_rep4	Biological replicate 4
PRJNA884180	SRX17748898	5.869895	var. secalinum Yablochny	vascular bundle		Vas_S1_rep1	Biological replicate 1
PRJNA884180	SRX17748899	10.145826	var. secalinum Yablochny	vascular bundle		Vas_S1_rep2	Biological replicate 2
PRJNA884180	SRX17748900	5.766617	var. secalinum Yablochny	vascular bundle		Vas_S1_rep3	Biological replicate 3
PRJNA884180	SRX17748901	3.496381	var. secalinum Yablochny	vascular bundle		Vas_S1_rep4	Biological replicate 4
PRJNA884180	SRX17749129	31.58453	var. secalinum Yablochny	part of the celery petiole without collenchyma strands		Collenchyma-free_S1_rep1	Biological replicate 1
PRJNA884180	SRX17749130	11.701432	var. secalinum Yablochny	part of the celery petiole without collenchyma strands		Collenchyma-free_S1_rep2	Biological replicate 2
PRJNA884180	SRX17749131	2.224211	var. secalinum Yablochny	part of the celery petiole without collenchyma strands		Collenchyma-free_S1_rep3	Biological replicate 3
PRJNA884180	SRX17749132	10.849224	var. secalinum Yablochny	part of the celery petiole without collenchyma strands		Collenchyma-free_S1_rep4	Biological replicate 4
PRJNA1124269	SRX24936411	0.938804		leaf		CK1	replicate=biological replicate1
PRJNA1124269	SRX24936412	0.063138		leaf		CK2	replicate=biological replicate2
PRJNA1124269	SRX24936413	0.12931		leaf		CK3	replicate=biological replicate3
PRJNA1124269	SRX24936414	0		leaf		D1	replicate=biological replicate1
PRJNA1124269	SRX24936415	1.712335		leaf		D2	replicate=biological replicate2
PRJNA1124269	SRX24936416	0.021898		leaf		D3	replicate=biological replicate3
PRJNA1124269	SRX24936417	0.196064		leaf		MD1	replicate=biological replicate1
PRJNA1124269	SRX24936418	1.159724		leaf		MD2	replicate=biological replicate2
PRJNA1124269	SRX24936419	0.424313		leaf		MD3	replicate=biological replicate3
PRJNA387092	SRX2833309	3.847599	shanghaihuangxin	leaf	leaves between 0.5 and 1 cm in breadth, folded;	stage 2
PRJNA387092	SRX2833321	0.702365	shanghaihuangxin	leaf	leaves between 2and 2.5 cm in breadth, nearly unfolded, with clear stem exten-sion	stage 5
PRJNA387092	SRX2833323	0.816959	shanghaihuangxin	leaf	leaves >3 cm, unfolded, with clearstem extension	stage 7
PRJNA543957	SRX5879598	9.028901	Zhangzhou lvqing	stem		Se3	100 ppm Na2SeO4
PRJNA543957	SRX5879599	1.066663	Zhangzhou lvqing	stem		Se2	100 ppm Na2SeO4
PRJNA543957	SRX5879600	16.246582	Zhangzhou lvqing	stem		Mock2	mock
PRJNA543957	SRX5879601	56.009872	Zhangzhou lvqing	stem		Mock1	mock
PRJNA543957	SRX5879602	7.799271	Zhangzhou lvqing	stem		Se1	100 ppm Na2SeO4
PRJNA543957	SRX5879603	2.003714	Zhangzhou lvqing	stem		Mock3	mock
PRJNA609149	SRX7815917	10.917325	Jinnan Shiqin	flower bud	early flower bud	early flower bud
PRJNA609149	SRX7815918	13.195496	Jinnan Shiqin	flower bud	middle flower bud	middle flower bud
PRJNA609149	SRX7815919	13.336627	Jinnan Shiqin	flower bud	early flowering period	early flowering period

Network

🔍 Co-expression Network

🔍 Protein-Protein Interaction Network

Orthology

Family	Species	Count	Orthologous Genes
Acanthaceae	Avicennia marina	8	jg28255, jg28388, jg3112, jg32942, jg35691, jg36492, jg36904 ... jg511
Aizoaceae	Mesembryanthemum crystallinum	5	gene_10373, gene_10948, gene_21402, gene_2204, gene_5986
Amaranthaceae	Atriplex hortensis	6	Ah004683, Ah005652, Ah007645, Ah007650, Ah015102, Ah026211
Amaranthaceae	Beta vulgaris	4	BVRB_4g076650, BVRB_4g085250, BVRB_5g108290, BVRB_9g219470
Amaranthaceae	Salicornia bigelovii	17	Sbi_jg11581, Sbi_jg11582, Sbi_jg11583, Sbi_jg11584 ... Sbi_jg17232, Sbi_jg19109, Sbi_jg19110, Sbi_jg24320, Sbi_jg24683, Sbi_jg24684, Sbi_jg37535, Sbi_jg41810, Sbi_jg46522, Sbi_jg61848, Sbi_jg61849, Sbi_jg8151, Sbi_jg8541
Amaranthaceae	Salicornia europaea	5	Seu_jg11870, Seu_jg20055, Seu_jg20834, Seu_jg491, Seu_jg5377
Amaranthaceae	Suaeda aralocaspica	5	GOSA_00001730, GOSA_00005158, GOSA_00005159, GOSA_00015416 ... GOSA_00022721
Amaranthaceae	Suaeda glauca	15	Sgl21220, Sgl21221, Sgl24395, Sgl26558, Sgl26559, Sgl26564 ... Sgl26565, Sgl26566, Sgl29570, Sgl42783, Sgl47537, Sgl52510, Sgl56386, Sgl57760, Sgl61917
Amaranthaceae	Chenopodium album	15	gene:ENSEOMG00000007815, gene:ENSEOMG00000009554 ... gene:ENSEOMG00000011352, gene:ENSEOMG00000012114, gene:ENSEOMG00000015846, gene:ENSEOMG00000022572, gene:ENSEOMG00000023795, gene:ENSEOMG00000024703, gene:ENSEOMG00000027272, gene:ENSEOMG00000035632, gene:ENSEOMG00000038441, gene:ENSEOMG00000042429, gene:ENSEOMG00000043166, gene:ENSEOMG00000044456, gene:ENSEOMG00000052042
Amaranthaceae	Chenopodium quinoa	9	CQ.Regalona.r1.4AG0007300, CQ.Regalona.r1.4BG0007530 ... CQ.Regalona.r1.4BG0018000, CQ.Regalona.r1.5AG0000910, CQ.Regalona.r1.5BG0010520, CQ.Regalona.r1.7BG0009780, CQ.Regalona.r1.8AG0016250, CQ.Regalona.r1.8BG0017330, CQ.Regalona.r1.9AG0014850
Anacardiaceae	Pistacia vera	9	pistato.v30020930, pistato.v30082040, pistato.v30082220 ... pistato.v30082310, pistato.v30082330, pistato.v30101500, pistato.v30101510, pistato.v30131840, pistato.v30211750
Apiaceae	Apium graveolens	6	Ag6G01868, Ag6G02028, Ag7G00134, Ag7G00136, Ag7G00222 ... Ag8G00366
Arecaceae	Cocos nucifera	5	COCNU_01G001480, COCNU_03G004660, COCNU_07G015560 ... COCNU_15G002740, scaffold023055G000040
Arecaceae	Phoenix dactylifera	6	gene-LOC103697005, gene-LOC103701408, gene-LOC103702179 ... gene-LOC103709371, gene-LOC103716081, gene-LOC120108368
Asparagaceae	Asparagus officinalis	8	AsparagusV1_02.2169.V1.1, AsparagusV1_04.1057.V1.1 ... AsparagusV1_05.2249.V1.1, AsparagusV1_07.771.V1.1, AsparagusV1_08.2684.V1.1, AsparagusV1_08.2685.V1.1, AsparagusV1_08.2792.V1.1, AsparagusV1_09.722.V1.1
Asteraceae	Flaveria trinervia	6	Ftri12G31397, Ftri14G02097, Ftri14G26220, Ftri15G18536 ... Ftri3G15988, Ftri4G04924
Brassicaceae	Arabidopsis thaliana	5	AT1G10930.1, AT1G31360.1, AT1G60930.1, AT3G05740.1 ... AT4G35740.1
Brassicaceae	Eutrema salsugineum	5	Thhalv10006602m.g.v1.0, Thhalv10006954m.g.v1.0 ... Thhalv10020334m.g.v1.0, Thhalv10023226m.g.v1.0, Thhalv10024545m.g.v1.0
Brassicaceae	Schrenkiella parvula	5	Sp1g09640.v2.2, Sp1g27200.v2.2, Sp2g03660.v2.2 ... Sp3g04620.v2.2, Sp7g33500.v2.2
Brassicaceae	Brassica nigra	6	BniB01g057930.2N, BniB02g005920.2N, BniB03g004180.2N ... BniB03g013340.2N, BniB04g020590.2N, BniB07g019200.2N
Casuarinaceae	Casuarina equisetifolia	4	Ceq03G0024, Ceq06G1127, Ceq09G0389, Ceq09G0399
Casuarinaceae	Casuarina glauca	5	Cgl03G0030, Cgl06G0692, Cgl06G0793, Cgl06G1174, Cgl09G0429
Cymodoceaceae	Cymodocea nodosa	6	gene.Cymno04g13480, gene.Cymno05g13160, gene.Cymno07g01730 ... gene.Cymno08g03930, gene.Cymno09g01390, gene.Cymno18g04950
Dunaliellaceae	Dunaliella salina	7	Dusal.0020s00027.v1.0, Dusal.0020s00028.v1.0 ... Dusal.0020s00029.v1.0, Dusal.0117s00007.v1.0, Dusal.0209s00011.v1.0, Dusal.0312s00009.v1.0, Dusal.0312s00010.v1.0
Hydrocharitaceae	Thalassia testudinum	5	gene.Thate01g30250, gene.Thate02g06120, gene.Thate06g06100 ... gene.Thate06g09850, gene.Thate09g19510
Malvaceae	Hibiscus hamabo Siebold & Zucc.	1	nbisL1-mrna-8263
Nitrariaceae	Nitraria sibirica	5	evm.TU.LG01.509, evm.TU.LG02.2837, evm.TU.LG03.2526 ... evm.TU.LG05.926, evm.TU.LG08.757
Plantaginaceae	Plantago ovata	5	Pov_00001994, Pov_00003378, Pov_00003709, Pov_00036705 ... Pov_00039241
Plumbaginaceae	Limonium bicolor	7	Lb1G08105, Lb1G08107, Lb2G14222, Lb5G26915, Lb5G29560 ... Lb5G29606, Lb8G35696
Poaceae	Echinochloa crus-galli	13	AH03.4079, AH04.2158, AH07.331, AH07.3692, BH03.1658 ... BH04.2336, BH04.2341, BH07.3505, BH07.433, CH03.1812, CH03.4491, CH04.2578, CH07.3580
Poaceae	Eleusine coracana subsp. coracana	10	gene-QOZ80_2AG0101190, gene-QOZ80_2AG0148060 ... gene-QOZ80_2BG0154570, gene-QOZ80_2BG0203460, gene-QOZ80_2BG0203470, gene-QOZ80_7BG0613160, gene-QOZ80_9AG0671310, gene-QOZ80_9AG0692270, gene-QOZ80_9BG0694780, gene-QOZ80_9BG0718310
Poaceae	Hordeum vulgare	5	HORVU.MOREX.r3.2HG0111650.1, HORVU.MOREX.r3.2HG0169030.1 ... HORVU.MOREX.r3.4HG0354110.1, HORVU.MOREX.r3.4HG0362480.1, HORVU.MOREX.r3.6HG0620770.1
Poaceae	Lolium multiflorum	6	gene-QYE76_016504, gene-QYE76_025762, gene-QYE76_025763 ... gene-QYE76_044072, gene-QYE76_065337, gene-QYE76_065610
Poaceae	Oryza coarctata	9	Oco03G023420, Oco04G024000, Oco07G006170, Oco08G006110 ... Oco13G016010, Oco14G016830, Oco21G009650, Oco22G010950, Oco22G011660
Poaceae	Paspalum vaginatum	6	gene-BS78_01G266600, gene-BS78_02G392600, gene-BS78_K262600 ... gene-BS78_04G033600, gene-BS78_04G296900, gene-BS78_05G256200
Poaceae	Puccinellia tenuiflora	10	Pt_Chr0103464, Pt_Chr0103556, Pt_Chr0203032, Pt_Chr0206934 ... Pt_Chr0302632, Pt_Chr0302666, Pt_Chr0305440, Pt_Chr0305467, Pt_Chr0604974, Pt_Chr0604976
Poaceae	Sporobolus alterniflorus	12	Chr07G013990, Chr0G002400, Chr0G003660, Chr0G012030 ... Chr0G029140, Chr0G032350, Chr23G019700, Chr24G012260, Chr26G000220, Chr26G000230, Chr26G000240, Chr31G008460
Poaceae	Thinopyrum elongatum	5	Tel2E01G226200, Tel2E01G537900, Tel4E01G215000 ... Tel4E01G228200, Tel6E01G603000
Poaceae	Triticum dicoccoides	10	gene_TRIDC2AG012300, gene_TRIDC2AG044270 ... gene_TRIDC2BG015080, gene_TRIDC2BG046980, gene_TRIDC4AG027310, gene_TRIDC4AG028650, gene_TRIDC4BG019850, gene_TRIDC4BG021710, gene_TRIDC6AG050960, gene_TRIDC6BG059650
Poaceae	Triticum aestivum	15	TraesCS2A02G103300.1, TraesCS2A02G304900.1 ... TraesCS2B02G120600.1, TraesCS2B02G321700.1, TraesCS2D02G102800.1, TraesCS2D02G303500.1, TraesCS4A02G170100.1, TraesCS4A02G178600.5, TraesCS4B02G126600.1, TraesCS4B02G138800.1, TraesCS4D02G133500.1, TraesCS4D02G144400.2, TraesCS6A02G339900.1, TraesCS6B02G371000.2, TraesCS6D02G320500.1
Poaceae	Zea mays	7	Zm00001eb026880_P004, Zm00001eb165270_P001 ... Zm00001eb231860_P001, Zm00001eb239640_P001, Zm00001eb257010_P001, Zm00001eb330800_P002, Zm00001eb431670_P001
Poaceae	Zoysia japonica	6	nbis-gene-33262, nbis-gene-33765, nbis-gene-45943 ... nbis-gene-45945, nbis-gene-45999, nbis-gene-8520
Poaceae	Zoysia macrostachya	6	Zma_g12171, Zma_g14558, Zma_g14559, Zma_g19176, Zma_g24573 ... Zma_g7958
Portulacaceae	Portulaca oleracea	12	evm.TU.LG01.469, evm.TU.LG02.2474, evm.TU.LG02.2475 ... evm.TU.LG04.291, evm.TU.LG06.1256, evm.TU.LG08.742, evm.TU.LG10.556, evm.TU.LG11.604, evm.TU.LG17.567, evm.TU.LG22.1186, evm.TU.LG23.694, evm.TU.LG23.695
Posidoniaceae	Posidonia oceanica	6	gene.Posoc01g28100, gene.Posoc01g30810, gene.Posoc05g11510 ... gene.Posoc05g19370, gene.Posoc07g02060, gene.Posoc08g03910
Rhizophoraceae	Bruguiera sexangula	4	evm.TU.Scaffold_14_RagTag.743, evm.TU.Scaffold_1_RagTag.73 ... evm.TU.Scaffold_1_RagTag.787, evm.TU.Scaffold_6_RagTag.2163
Rhizophoraceae	Carallia pectinifolia	4	nbisL1-mrna-16970, nbisL1-mrna-17701, nbisL1-mrna-7310 ... nbisL1-mrna-8202
Rhizophoraceae	Ceriops tagal	4	nbisL1-mrna-12996, nbisL1-mrna-13389, nbisL1-mrna-17648 ... nbisL1-mrna-5172
Rhizophoraceae	Ceriops zippeliana	5	nbisL1-mrna-11667, nbisL1-mrna-11668, nbisL1-mrna-17086 ... nbisL1-mrna-5159, nbisL1-mrna-5799
Rhizophoraceae	Kandelia candel	4	evm.TU.utg000007l.8, evm.TU.utg000011l.1329 ... evm.TU.utg000012l.50, evm.TU.utg000012l.504
Rhizophoraceae	Kandelia obovata	4	Maker00007012, Maker00011357, Maker00011494, Maker00015978
Rhizophoraceae	Rhizophora apiculata	5	nbisL1-mrna-15706, nbisL1-mrna-15707, nbisL1-mrna-15843 ... nbisL1-mrna-7908, nbisL1-mrna-8301
Rhizophoraceae	Rhizophora mangle	5	nbisL1-mrna-2951, nbisL1-mrna-67, nbisL1-mrna-897 ... nbisL1-mrna-9019, nbisL1-mrna-9020
Salicaceae	Populus euphratica	7	populus_peu13308, populus_peu14072, populus_peu17048 ... populus_peu17049, populus_peu33352, populus_peu33356, populus_peu34153
Solanaceae	Lycium barbarum	7	gene-LOC132598421, gene-LOC132598678, gene-LOC132614398 ... gene-LOC132616885, gene-LOC132635550, gene-LOC132635553, gene-LOC132644378
Solanaceae	Solanum chilense	5	SOLCI000829600, SOLCI001635100, SOLCI002212700 ... SOLCI002906700, SOLCI003190000
Solanaceae	Solanum pennellii	5	gene-LOC107012022, gene-LOC107020301, gene-LOC107024232 ... gene-LOC107029016, gene-LOC107031384
Tamaricaceae	Reaumuria soongarica	6	STRG.14383_chr08_-, gene_10751, gene_16133, gene_3038 ... gene_6451, gene_8163
Tamaricaceae	Tamarix chinensis	7	TC01G1360, TC01G1361, TC09G0552, TC10G1035, TC10G1036 ... TC11G2030, TC11G2416
Zosteraceae	Zostera marina	6	Zosma01g27830.v3.1, Zosma01g28950.v3.1, Zosma03g06920.v3.1 ... Zosma05g02290.v3.1, Zosma05g16550.v3.1, Zosma05g22360.v3.1